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| Urios, Laurent; Agogue, Hélène; Lesongeur, Francoise; Stackebrandt, Erko; Lebaron, Philippe. |
| A novel aerobic, Gram-negative bacterium, named 13IX/A01/164(T), was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were motile, straight rods, 2-5 mu m long and 0-2 mu m wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164T was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum Bacteroidetes within the family Crenotrichaceae. On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name Balneola vulgaris gen. nov., sp. nov. is proposed. The type strain of Balneola vulgaris is 13IX/A01/164T (=DSM 17893T=CIP... |
| Tipo: Text |
Palavras-chave: Mediterranean sea; Coastal waters; Microbiology; Physiology; Biochemistry; Gene sequence; Balneola vulgaris. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2126.pdf |
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| Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne. |
| The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture... |
| Tipo: Text |
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf |
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| Urios, Laurent; Cueff, Valerie; Pignet, Patricia; Barbier, Georges. |
| A novel anaerobic, Gram-positive, sporulating and strictly chemoorganoheterotrophic bacterium, designated strain DV1 184 T, was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were short, straight rods (4 mum long and 0.8 mum wide) and were motile with peritrichous flagella. They grew between 35 and 55 degreesC (optimum, 45 degreesC), between pH 5.0 and 8.0 (optimum, 6.0) and at 20-60 g sea salts l(-1) (optimum sea salts concentration, 30 g l(-1)). Strain DV1184(T) was able to ferment yeast extract, tryptone, peptone, glucose, sucrose, maltose and pyruvate. The main fermentation products from glucose were (in decreasing order) formate, acetate and ethanol. The genomic DNA G+C content was 29 mol%. Phylogenetic... |
| Tipo: Text |
Palavras-chave: Phylogeny; Bacteria; Deep sea hydrothermal vent. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-589.pdf |
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| Urios, Laurent; Michotey, Valerie; Intertaglia, Laurent; Lesongeur, Francoise; Lebaron, Philippe. |
| A novel Gram-negative bacteria, named CZ41_10a(T), was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 mu m long and 0.7 mu m wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus Thalassobaculum, a member of the family Rhodospirillaceae, class Alphaproteobacteria. Unlike Thalassobaculum litoreum CL-GR58(T), its closest relative, strain CZ41_10a(T) was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA-DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00001/11201/7846.pdf |
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| Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges. |
| A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the... |
| Tipo: Text |
Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf |
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